@article {1724,
	title = { Biochemical characterization of the skeletal matrix of the massive coral, Porites australiensis - The saccharide moieties and their localization },
	journal = { JOURNAL OF STRUCTURAL BIOLOGY },
	volume = {203},
	year = {2018},
	pages = {219-229},
	abstract = {<p>To construct calcium carbonate skeletons of sophisticated architecture, scleractinian corals secrete an extra cellular skeletal organic matrix (SOM) from aboral ectodermal cells. The SOM, which is composed of proteins, saccharides, and lipids, performs functions critical for skeleton formation. Even though polysaccharides constitute the major component of the SOM, its contribution to coral skeleton formation is poorly understood. To this end, we analyzed the SOM of the massive colonial coral, Porites australiensis, the skeleton of which has drawn great research interest because it records environmental conditions throughout the life of the colony. The coral skeleton was extensively cleaned, decalcified with acetic acid, and organic fractions were separated based on solubility. These fractions were analyzed using various techniques, including SDS-PAGE, FT-IR, in vitro crystallization, CHNS analysis, chromatography analysis of monosaccharide and enzyme-linked lectin assay (ELLA). We confirmed the acidic nature of SOM and the presence of sulphate, which is thought to initiate CaCO3 crystallization. In order to analyze glycan structures, we performed ELLA on the soluble SOM for the first time and found that it exhibits strong specificity to Datura stramonium lectin (DSL). Furthermore, using biotinylated DSL with anti-biotin antibody conjugated to nanogold, in situ localization of DSL-binding polysaccharides in the P. australiensis skeleton was performed. Signals were distributed on the surfaces of fiber-like crystals of the skeleton, suggesting that polysaccharides may modulate crystal shape. Our study emphasizes the importance of sugar moieties in biomineralization of scleractinian corals.</p>},
	url = {https://hal.archives-ouvertes.fr/hal-02122753/},
	author = {T. TAKEUCHI and L. PLASSERAUD and I. ZIEGLER-DEVIN and N. BROSSE and C. SHINZATO and N. SATOH and F. MARIN}
}
