@article {1856, title = { Mining the Biosynthetic Potential for Specialized Metabolism of a Streptomyces Soil Community }, journal = { ANTIBIOTICS-BASEL }, volume = {9}, year = {2020}, pages = {271}, abstract = {

The diversity and distribution of specialized metabolite gene clusters within a community of bacteria living in the same soil habitat are poorly documented. Here we analyzed the genomes of 8 Streptomyces isolated at micro-scale from a forest soil that belong to the same species or to different species. The results reveal high levels of diversity, with a total of 261 biosynthesis gene clusters (BGCs) encoding metabolites such as terpenes, polyketides (PKs), non-ribosomal peptides (NRPs) and ribosomally synthesized and post-translationally modified peptides (RiPPs) with potential bioactivities. A significant part of these BGCs (n = 53) were unique to only one strain when only 5 were common to all strains. The metabolites belong to very diverse chemical families and revealed that a large diversity of metabolites can potentially be produced in the community. Although that analysis of the global metabolome using GC-MS revealed that most of the metabolites were shared between the strains, they exhibited a specific metabolic pattern. We also observed that the presence of these accessory pathways might result from frequent loss and gain of genes (horizontal transfer), showing that the potential of metabolite production is a dynamic phenomenon in the community. Sampling Streptomyces at the community level constitutes a good frame to discover new biosynthetic pathways and it appears as a promising reservoir for the discovery of new bioactive compounds.

}, url = {https://hal.univ-lorraine.fr/hal-02619232}, author = {M. NICAULT and A.R. TIDJANI and A. GAUTHIER and S. DUMARCAY and E. GELHAYE and C. BONTEMPS and P. LEBLOND} } @article {1869, title = { A reverse chemical ecology approach to explore wood natural durability }, journal = { MICROBIAL BIOTECHNOLOGY }, year = {2020}, abstract = {

The natural durability of wood species, defined as their inherent resistance to wood-destroying agents, is a complex phenomenon depending on many biotic and abiotic factors. Besides the presence of recalcitrant polymers, the presence of compounds with antimicrobial properties is known to be important to explain wood durability. Based on the advancement in our understanding of fungal detoxification systems, a reverse chemical ecology approach was proposed to explore wood natural durability using fungal glutathione transferases. A set of six glutathione transferases from the white-rot Trametes versicolor were used as targets to test wood extracts from seventeen French Guiana neotropical species. Fluorescent thermal shift assays quantified interactions between fungal glutathione transferases and these extracts. From these data, a model combining this approach and wood density significantly predicts the wood natural durability of the species tested previously using long-term soil bed tests. Overall, our findings confirm that detoxification systems could be used to explore the chemical environment encountered by wood-decaying fungi and also wood natural durability.

}, author = {T. PERROT and G. SALZET and N. AMUSANT and J. BEAUCHENE and P. GERARDIN and S. DUMARCAY and R. SORMANI and M. MOREL-ROUHIER and E. GELHAYE} } @article {1746, title = { Comparison of teak wood properties according to forest management: short versus long rotation }, journal = { ANNALS OF FOREST SCIENCE }, volume = {75}, year = {2018}, abstract = {

Key message Teak (Tectona grandis L.f.) is one of the most important tropical hardwood tree species, which is widely planted in Indonesia. Wood properties are strongly influenced by forest management conditioning further utilization of wood.

Context In Indonesia, teak wood has been supplied from the state forests (Perhutani) for long rotation teak and from community teak plantations for short rotation teak. Short rotation teak has been harvested at 7-10 years and long rotation teak at 40-60 years.

Aims This paper discusses the characterization of technical properties of short and long rotation teak wood based on the chemical, anatomical, physical, and mechanical properties.

Methods The properties of short rotation and long rotation teak woods were characterized by measuring their density, extractive contents, chemical composition, swelling, wettability, water sorption isotherm, decay resistance, anatomical properties, bending strength (modulus of rupture (MOR), modulus of elasticity (MOE)), and hardness.

Results The results indicate that short rotation teak was not particularly different in swelling, MOE and MOR, and Brinell hardness compared to long rotation teak, although it was less dense and less durable due to lower heartwood and extractive contents. Therefore, careful attention should be given to the use of short rotation teak in some wood-processing technologies.

Conclusion Lower wood density and durability of the short rotation compared to the long rotation teak will restrict its utilization to some extent for both indoor and outdoor applications. Fast-growing teak from community cannot be used as usual heartwood teak from Perhutani because of the very low proportion of useful heartwood in the stem.

}, author = {D.E. RIZANTI and W. DARMAWAN and B. GEORGE and A. MERLIN and S. DUMARCAY and H. CHAPUIS and C. GERARDIN and E. GELHAYE and P. RAHARIVELOMANANA and R.K. SARI and W. SYAFII and R. MOHAMED and P. GERARDIN} } @article {1721, title = { Fungal Glutathione Transferases as Tools to Explore the Chemical Diversity of Amazonian Wood Extractives }, journal = { ACS SUSTAINABLE CHEMISTRY \& ENGINEERING }, volume = {6}, year = {2018}, pages = {13078-13085}, abstract = {

The natural durability of wood is linked to its chemical composition and in particular the presence of metabolites called extractives that often possess chemical reactivity. For dealing with these compounds, wood degraders have developed detoxification systems usually involving enzyme families. Among these enzymes, glutathione transferases (GSTs) are involved in the decrease of the reactivity of toxic compounds. In this study, the hypothesis that the detoxification systems of wood decaying fungi could be indicators of the chemical reactivity of wood extracts has been tested. This approach has been evaluated using 32 wood extracts coming from French Guiana species, testing their antimicrobial ability, antioxidative properties, and reactivity against six GSTs from the white rot Trametes versicolor. From the obtained data, a significant correlation between the antimicrobial and antioxidative properties of the tested wood extracts and GST interactions was established. In addition, the chemical analysis performed on one of the most reactive extracts (an acetonic extract of Bagassa guianensis) has demonstrated oxyresveratrol as a major constituent. We were able to cocrystallize one GST with this commercially interesting compound. Taken together, the presented data support the hypothesis that detoxifying enzymes could be used to identify the presence of molecules of industrial interest in wood extracts.

}, url = {https://hal.archives-ouvertes.fr/hal-02492224}, author = {T. PERROT and M. SCHWARTZ and F. SAIAG and G. SALZET and S. DUMARCAY and F. FAVIER and P. GERARDIN and J.M. GIRARDET and R. SORMANI and M. MOREL-ROUHIER and N. AMUSANT and C. DIDIERJEAN and E. GELHAYE} } @article {1737, title = { Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor }, journal = { SCIENTIFIC REPORTS }, volume = {8}, year = {2018}, abstract = {

Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.

}, url = {https://hal.univ-lorraine.fr/LERMAB-UL/hal-01843732}, author = {M. SCHWARTZ and T. PERROT and E. AUBERT and S. DUMARCAY and F. FAVIER and P. GERARDIN and M. MOREL-ROUHIER and G. MULLIERT and F. SAIAG and C. DIDIERJEAN and E. GELHAYE} } @article {1628, title = { Total phenolic and lignin contents, phytochemical screening, antioxidant and fungal inhibition properties of the heartwood extractives of ten Congo Basin tree species }, journal = { ANNALS OF FOREST SCIENCE }, volume = {73}, year = {2016}, pages = {287-296}, abstract = {

Content and type of extractives vary considerably among woody species in the Congo Basin tropical forest. They influence the natural durability of wood with respect to fungal and insect degradation. Species containing higher amounts of extractives and Klason lignin were generally less susceptible to fungal colonization. Phenolic extractives with large anti-fungal and high antioxidant activities were the main chemical family of compounds extracted from the tested species, which suggests a potential chemical valorization of these extractives.

Several woody species from the Congo Basin are known for their natural durability, but the causes of this natural durability are not always fully elucidated. This is particularly the case for the resistance to white rotting fungi decay.

The chemical composition as well as the antioxidant activity of the heartwood extractives of several species were quantified and correlated to the ability of different fungi to colonize wood sawdusts in order to better understand their decay resistance.

The chemical screening of extracts was conducted using colorimetric methods. The quantity of lignin, extracts and total phenolic compounds, as well as antioxidant activities were determined. Extracted and unextracted heartwood sawdusts were exposed to two white rot fungi to assess the effect of extractives on their colonization.

Extractives and lignin contents ranged from 2.7 to 16.0 \% and from 26.5 to 35.9 \%, respectively. Antioxidant activity was directly connected to total phenolic contents. The phytochemical screening revealed the presence of different chemical groups in all extracts. Resistance to fungi was correlated to the amount of extractives and in most cases to the lignin content.

Phenolic compounds associated with antioxidant activities influenced the natural resistance of heartwood.

}, url = {https://hal.univ-lorraine.fr/LERMAB-UL/hal-01532273}, author = {S. MOUNGUENGUI and J.B.S. TCHINDA and M.K. NDIKONTAR and S. DUMARCAY and C. ATTEKE and D. PERRIN and E. GELHAYE and P. GERARDIN} } @article {1396, title = {The GSTome reflects the chemical environment of white-rot fungi.}, year = {2015}, author = {A. DEROY and F. SAIAG and Z. KEBBI-BENKEDER and N. TOUAHRI and A. HECKER and M. MOREL-ROUHIER and F. COLIN and S. DUMARCAY and P. GERARDIN and E. GELHAYE} } @article {1206, title = {Transcriptomic responses of Phanerochaete chrysosporium to wood extractives: focus on a new glutathione transferase. }, journal = {Appl. Environ. Microbiol. }, volume = {80}, year = {2014}, month = {2014}, chapter = {6316}, author = {A. THUILLIER and K. CHIBANI and G. BELLI and E. HERRERO and S. DUMARCAY and P. GERARDIN and A. KOHLER and A. DEROY and T. DHALLEINE and R. BCHINI and J.P. JACQUOT and E. GELHAYE and M. MOREL-ROUHIER} } @article {612, title = {New substrates and activity of Phanerochaete chrysosporium Omega glutathione transferases.}, journal = {Biochimie}, volume = {95}, year = {2013}, month = {2013}, chapter = {336-346}, author = {E. MEUX and M. MOREL and T. LAMANT and P. GERARDIN and JP. JACQUOT and S. DUMARCAY and E. GELHAYE} } @article {623, title = {Selection and validation of enzymatic activities as functional markers in wood biotechnology and fungal ecology}, journal = {Journal of Microbiological Methods }, volume = {92}, year = {2013}, month = {2013}, chapter = {152-163}, author = {Y. MATHIEU and E. GELHAYE and S. DUMARCAY and P. GERARDIN and L. HARVENGT and M. BUEE} } @article {557, title = {Characterization of a Phanerochaete chrysosporium Glutathione Transferase Reveals a Novel Structural and Functional Class with Ligandin Properties}, journal = {Journal of Biological Chemistry}, volume = {287}, year = {2012}, month = {2012}, chapter = {39001-39011}, author = {Y. MATHIEU and P. PROSPER and M. BUEE and S. DUMARCAY and F. FAVIER and E. GELHAYE and P. GERARDIN and L. HARVENGT and J-P. JACQUOT and T. LAMANT} } @article {555, title = {Sphingobium sp. SYK-6 LigG involved in lignin degradation is structurally and biochemically related to the glutathione transferase omega class}, journal = {FEBS Letters }, volume = {586}, year = {2012}, month = {2012}, chapter = {29-39}, author = {E. MEUX and P. PROSPER and E. MASAI and G. MULLIERT and S. DUMARCAY and M. MOREL and C. DIDIERJEAN and E. GELHAYE and F. FAVIER} }